Composite

Part:BBa_K3909018:Design

Designed by: Xixi Song   Group: iGEM21_Worldshaper-Nanjing   (2021-10-14)


pYLXP-ylPOT1-ylMFE1-ylPOX1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1343
    Illegal BamHI site found at 6611
    Illegal BamHI site found at 6822
    Illegal XhoI site found at 164
    Illegal XhoI site found at 565
    Illegal XhoI site found at 2378
    Illegal XhoI site found at 2840
    Illegal XhoI site found at 4249
    Illegal XhoI site found at 4341
    Illegal XhoI site found at 5161
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 832
    Illegal NgoMIV site found at 925
    Illegal NgoMIV site found at 1159
    Illegal NgoMIV site found at 7653
    Illegal NgoMIV site found at 7705
    Illegal AgeI site found at 1657
    Illegal AgeI site found at 1741
    Illegal AgeI site found at 1893
    Illegal AgeI site found at 2791
    Illegal AgeI site found at 4410
    Illegal AgeI site found at 5115
    Illegal AgeI site found at 5642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2806
    Illegal BsaI site found at 4746
    Illegal BsaI site found at 5301
    Illegal BsaI site found at 7337
    Illegal BsaI.rc site found at 2
    Illegal BsaI.rc site found at 844
    Illegal BsaI.rc site found at 2216
    Illegal BsaI.rc site found at 4723
    Illegal BsaI.rc site found at 5400
    Illegal BsaI.rc site found at 5965
    Illegal SapI site found at 4346
    Illegal SapI site found at 5501


Design Notes

1


Source

1

References